yap activator Search Results


yap crispr activation plasmids  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology yap crispr activation plasmids
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Yap Crispr Activation Plasmids, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pmc09550503-39-9-17?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
yap crispr activation plasmids - by Bioz Stars, 2026-08
93/100 stars
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yap/taz localization  (DuPont de Nemours)
90
DuPont de Nemours yap/taz localization
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Yap/Taz Localization, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pmc06815268-90-4-24?v=DuPont+de+Nemours
Average 90 stars, based on 1 article reviews
yap/taz localization - by Bioz Stars, 2026-08
90/100 stars
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yes-associated protein (yap) activation  (CH Instruments)
90
CH Instruments yes-associated protein (yap) activation
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Yes Associated Protein (Yap) Activation, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm37260407-167-11-18?v=CH+Instruments
Average 90 stars, based on 1 article reviews
yes-associated protein (yap) activation - by Bioz Stars, 2026-08
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activity of mammalian yki homologues yap and taz  (DuPont de Nemours)
90
DuPont de Nemours activity of mammalian yki homologues yap and taz
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Activity Of Mammalian Yki Homologues Yap And Taz, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm23239028-272-15-21?v=DuPont+de+Nemours
Average 90 stars, based on 1 article reviews
activity of mammalian yki homologues yap and taz - by Bioz Stars, 2026-08
90/100 stars
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flagtagged constitutively active yap[5sa] viruses  (VectorBuilder GmbH)
90
VectorBuilder GmbH flagtagged constitutively active yap[5sa] viruses
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Flagtagged Constitutively Active Yap[5sa] Viruses, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm33847835-83-3-17?v=VectorBuilder+GmbH
Average 90 stars, based on 1 article reviews
flagtagged constitutively active yap[5sa] viruses - by Bioz Stars, 2026-08
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nuclear (transcrip- tionally active) yap  (TranScrip Partners)
90
TranScrip Partners nuclear (transcrip- tionally active) yap
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Nuclear (Transcrip Tionally Active) Yap, supplied by TranScrip Partners, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm39451413-167-10-8?v=TranScrip+Partners
Average 90 stars, based on 1 article reviews
nuclear (transcrip- tionally active) yap - by Bioz Stars, 2026-08
90/100 stars
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constitutively-active yap mutant yap5sa  (DuPont de Nemours)
90
DuPont de Nemours constitutively-active yap mutant yap5sa
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Constitutively Active Yap Mutant Yap5sa, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pmc08040027-199-6-20?v=DuPont+de+Nemours
Average 90 stars, based on 1 article reviews
constitutively-active yap mutant yap5sa - by Bioz Stars, 2026-08
90/100 stars
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active yap/taz  (DuPont de Nemours)
90
DuPont de Nemours active yap/taz
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Active Yap/Taz, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm37909406-120-1-10?v=DuPont+de+Nemours
Average 90 stars, based on 1 article reviews
active yap/taz - by Bioz Stars, 2026-08
90/100 stars
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yes-associated protein (yap)/transcriptional co-activator with pdz-binding motif (taz)  (Enzo Biochem)
90
Enzo Biochem yes-associated protein (yap)/transcriptional co-activator with pdz-binding motif (taz)
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Yes Associated Protein (Yap)/Transcriptional Co Activator With Pdz Binding Motif (Taz), supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm31433973-150-9-21?v=Enzo+Biochem
Average 90 stars, based on 1 article reviews
yes-associated protein (yap)/transcriptional co-activator with pdz-binding motif (taz) - by Bioz Stars, 2026-08
90/100 stars
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yes-associated protein/transcription co-activator (yap/taz)  (Enzo Biochem)
90
Enzo Biochem yes-associated protein/transcription co-activator (yap/taz)
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Yes Associated Protein/Transcription Co Activator (Yap/Taz), supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm35849943-131-8-15?v=Enzo+Biochem
Average 90 stars, based on 1 article reviews
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activated yap  (DuPont de Nemours)
90
DuPont de Nemours activated yap
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Activated Yap, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm24806962-156-9-30?v=DuPont+de+Nemours
Average 90 stars, based on 1 article reviews
activated yap - by Bioz Stars, 2026-08
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constitutively active yap (5sa) dna  (Cyagen Biosciences)
90
Cyagen Biosciences constitutively active yap (5sa) dna
Conditioned medium from <t>YAP</t> overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP <t>CRISPR/Cas9</t> KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.
Constitutively Active Yap (5sa) Dna, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+activator/pm38245803-68-6-13?v=Cyagen+Biosciences
Average 90 stars, based on 1 article reviews
constitutively active yap (5sa) dna - by Bioz Stars, 2026-08
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Image Search Results


Conditioned medium from YAP overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP CRISPR/Cas9 KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells

doi: 10.1155/2022/5942379

Figure Lengend Snippet: Conditioned medium from YAP overexpressed breast cancer cells promoted tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). (a) MDA-MB-231 cells were transfected with YAP CRISPR/Cas9 KO plasmid (YAP KD) and YAP CRISPR activation plasmids (YAP OE), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium obtained from YAP knock down (CM-YAP-) and overexpressed (CM-YAP+) breast cancer cells for 12 hr. The total segment length and total branching length from tube formation assay are quantified with means ± SD from six independent representative fields. ∗∗∗ p < 0.001. (c) Migration of HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (d) HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. ∗∗ p < 0.01. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Cells were transfected with YAP CRISPR/Cas9 KO plasmid and YAP CRISPR activation plasmids using UltraCruz® Transfection Reagent (Santa Cruz, USA) according to manufacturer's instruction.

Techniques: Migration, Transfection, CRISPR, Plasmid Preparation, Activation Assay, Expressing, Western Blot, Knockdown, Tube Formation Assay, Transwell Assay, Cloning

YAP expression in HUVECs involved in mediating CM-YAP+ induced tube formation, migration, and proliferation of HUVECs. (a) HUVECs were transfected with YAP CRISPR/Cas9 KO plasmid (YAP KD), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, HUVECs, and YAP knock down (KD) HUVECs were treated with different conditioned medium obtained from breast cancer cells for 12 hr. The total segment length and total branching length are quantified with mean ± SD from six independent representative fields ∗ p < 0.05, ∗∗ p < 0.01. (c) Migration of HUVECs and YAP knock down HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments, ∗∗ p < 0.01. (d) HUVECs and YAP KD HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗∗ p < 0.01.

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells

doi: 10.1155/2022/5942379

Figure Lengend Snippet: YAP expression in HUVECs involved in mediating CM-YAP+ induced tube formation, migration, and proliferation of HUVECs. (a) HUVECs were transfected with YAP CRISPR/Cas9 KO plasmid (YAP KD), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, HUVECs, and YAP knock down (KD) HUVECs were treated with different conditioned medium obtained from breast cancer cells for 12 hr. The total segment length and total branching length are quantified with mean ± SD from six independent representative fields ∗ p < 0.05, ∗∗ p < 0.01. (c) Migration of HUVECs and YAP knock down HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments, ∗∗ p < 0.01. (d) HUVECs and YAP KD HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. The cloning formation rate is quantified with mean ± SD from six independent wells. ∗∗ p < 0.01.

Article Snippet: Cells were transfected with YAP CRISPR/Cas9 KO plasmid and YAP CRISPR activation plasmids using UltraCruz® Transfection Reagent (Santa Cruz, USA) according to manufacturer's instruction.

Techniques: Expressing, Migration, Transfection, CRISPR, Plasmid Preparation, Western Blot, Knockdown, Transwell Assay, Cloning